Figure 1. DEX exposure induces alteration in DNA methylation and hydroxymethylation. A: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed after 48 h of incubation at various concentrations of dexamethasone (DEX). No significant changes in cell viability were observed. B: Changes in the genomic DNA methylation level of ARPE-19 cells exposed to 0.5, 1.0, and 2.0 mg/ml DEX after 48 h. C: Changes in the genomic DNA hydroxymethylation level of ARPE-19 cells exposed to 0.5, 1.0, and 2.0 mg/ml of DEX after 48 h. *p<0.05 with the Student two-tailed t test compared to the control group. n=3.