Figure 3. IC₅₀ determination for inhibition of CYP4V2 by HET0016. Activity of CYP4V2 toward luciferin-3FEME was determined either without an inhibitor (with dimethyl sulfoxide (DMSO) as a vehicle control) or in the presence of HET0016 at final concentrations between 1 nM and 2 µM as indicated. Data shown were calculated from three independent experiments performed in triplicate.