Figure 1. Screening of luminogenic substrates for conversion by CYP4V2. Permeabilized fission yeast cells (enzyme bags) were used for the biotransformation of different luminogenic substrates as indicated. The activities of strains RAJ232 (coexpressing human CYP4V2 and CPR; black columns) and CAD62 (control strain expressing only CPR; gray columns) are shown in relative light units (RLUs). Data shown were calculated from three independent experiments performed in triplicate. For comparison, data for luciferin-ME and luciferin-H are also shown [10]. ****p<0.001.