Table 1 of
Aweidah, Mol Vis 2021; 27:588-600. Table 1 of
Aweidah, Mol Vis 2021; 27:588-600.
Table 1. Primers used for polymerase chain reaction (PCR) for each gene region.
| Gene | Location | Variant | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) |
|---|---|---|---|---|
| CNGB3 | Exon 1 | c.105_114delTCAGTCTCAG, p.(Gln36Lysfs*44) | ACTAGCTAAGGAGTTGCCTG | AATTAATGAAGATAAGCCCG |
| CNGB3 | Exon 4 | c.467C>T, p.(Ser156Phe) | AATCTGTATTCCACCAGCAC | CTCGTACCTTCCTGGATTAC |
| CNGB3 | Exon 6 | c.644–1G>C, p.(?) | CTCTGTAGAGGGTAGTGCC | TGCAGATAGCCAGTCAAAC |
| CNGB3 | Exon 6 | c.782A>G, p.(Asp261Gly) | CTCTGTAGAGGGTAGTGCC | TGCAGATAGCCAGTCAAAC |
| CNGB3 | Exon 6 | c.819delC, p.(Arg274Aspfs*5) | CTCTGTAGAGGGTAGTGCC | TGCAGATAGCCAGTCAAAC |
| CNGB3 | Exon 9 | c.1006G>T, p.(Glu336*) | GGAAACAGAGTTCTACTACATGC | TGACAGAGGCAGATAATAAGTC |
| CNGB3 | Exon 10 | c.1148delC, p.(Thr383Ilefs*13) | GCTGTATTTCAGAAACAACATGAATC | CATAAATTCATACAATGAAACAGAATG |
| CNGB3 | Intron 14 | c.1663–1205G>A, p.(?) | AGTCCCCTC CTAAGCCAAGT | TATGAGGCCCTTGGAACAC |
| CNGB3 | Intron 14 | c.1578+1G>A, p.(?) | TGTTATTGTAATAGGTATGGAGG | TCTAAAGAATAAGCCGTTGG |
| CNGB3 | Exon 18 | c.2328delC, p.(Arg777Glufs*52) | GTGGTGATCTTAGCCATTG | CTTTCGTTTCTCAAGGGTC |