Figure 3 of Jun, Mol Vis 2021; 27:575-587.


Figure 3. Chemerin/ChemR23 pathway–mediated intercellular adhesion molecule-1 (ICAM-1) expression. A-D: Western blot analysis of ChemR23, tumor necrosis factor-α receptor (TNFR), and ICAM-1 proteins with/without chemerin stimulation (10 nM for 24 h) or ChemR23 knockdown under low- and high-glucose conditions, normalized to tubulin. E: The expression of ICAM-1 in RRMECs detected by real-time quantitative PCR (RT-qPCR) under low-glucose condition (5.5 mM). F: The expression of ICAM-1 in rat primary retinal microvascular endothelial cells (RRMECs) detected by RT-qPCR under high-glucose conditions (30 mM). Data are shown as mean ± standard deviation (SD) of six replicates. *p<0.05, ***p<0.001. Abbreviations: NC, RRMECs transfected with empty vector; sh, RRMECs transfected with shRNA targeting ChemR23; ch, with chemerin stimulation (10 nM for 24 h); ch/sh, RRMECs transfected with shRNA targeting ChemR23 and chemerin stimulation (10 nM for 24 h).