Figure 2. The expression of tumor necrosis factor-α receptor (TNFR) and interleukin-6 receptor (IL-6R) was not affected by ChemR23 knockdown in rat primary retinal microvascular endothelial cells (RRMECs). A and C-E: Western blot analysis of ChemR23, TNFR, and IL-6R proteins, normalized to tubulin under low- (5.5 mM) and high-glucose (30 mM) conditions. B: The expression of ChemR23 in RRMECs detected by real-time quantitative PCR (RT-qPCR). Data are shown as mean ± standard deviation (SD) of six replicates. *p<0.05, **p<0.01, ***p<0.001. Abbreviations: NC, RRMECs transfected with empty vector; shChemR23, RRMECs transfected with shRNA targeting ChemR23.