Figure 1. High concentrations of glucose increased chemerin expression, intercellular adhesion molecule-1 (ICAM-1), and tumor necrosis factor-α receptor (TNFR), whereas they had no effect on ChemR23 or interleukin-6 receptor (IL-6R). A,B: Western blot analysis of the expression of ChemR23 under different glucose concentrations. C: Real-time quantitative PCR (RT-qPCR) showed the mRNA expression of ChemR23 under different glucose conditions. D: RT-qPCR showed the mRNA expression of chemerin under different glucose conditions. E-H: Western blot analysis of ICAM-1, TNFR, and IL-6R proteins, normalized to tubulin. I: The expression of ICAM-1 in rat primary retinal microvascular endothelial cells (RRMECs) detected by RT-qPCR. J: The expression of TNFR in RRMECs detected by RT-qPCR. K: The expression of IL-6R in RRMECs detected by RT-qPCR. Data are shown as mean ± standard deviation (SD) of six replicates. **p<0.01, ***p<0.001.