Table 1 of
Gowtham, Mol Vis 2021; 27:564-573. Table 1 of
Gowtham, Mol Vis 2021; 27:564-573.
Table 1. List of neurotransmitter metabolites studied, their respective precursor (Q1) to fragment ion (Q3) transitions, retention time, limit of detection (LOD), R2 values of standard calibration along with calibration range.
| S.no | Neurotransmitter metabolites | Q1→Q3 | Retention time (min) | LOD (ng/ml) | R2 | Calibration range (ng/ml) |
|---|---|---|---|---|---|---|
| 1 | Acetyl choline | 146.2→87.2/60.2 | 3.5 | 0.2 | 0.998 | 0.313–20 |
| 2 | GABA | 104.1→87.1/69.1 | 7.65 | 2.5 | 0.998 | 5–320 |
| 3 | Glutamate | 148.1→130.1/ 84.1 | 8.25 | 90 | 0.999 | 125–8000 |
| 4 | 1-methyl Histamine | 126.1→109.2 | 16.64 | 0.12 | 0.998 | 0.313–20 |
| 5 | Histamine | 112.2→95.1/83.1 | 15.4 | 0.12 | 0.996 | 0.313–20 |
| 6 | N-Acetyl serotonin | 219.1→160.1 | 2.91 | 0.09 | 0.994 | 0.19–12.5 |
| 7 | Serotonin | 177.1→160.1/132.1 | 9.12 | 0.04 | 0.995 | 0.19–12.5 |
| 8 | Dopamine | 154.2→137.2/119.1/91.1 | 10.2 | 0.03 | 0.995 | 0.19–12.5 |
| 9 | Adrenaline | 184.1→166.1 | 10.5 | 0.01 | 0.995 | 0.030–3.84 |
| 10 | DOPA | 198.1→181.1/152.1 | 12.6 | 0.25 | 0.992 | 0.39–12.5 |
| 11 | Noradrenaline | 152.1→135.1/107.1 | 12.7 | 0.06 | 0.999 | 0.19–6.25 |