Figure 4. Defective ^retDync1h1^−/− retina lamination and impaired ciliogenesis. A, B: Plastic sections of the central retina near the optic nerve of heterozygous control (left) and ^retDync1h1^−/− (right) mice at P6 and P8. Sections are stained with methylene blue-Azure II (Richardson’s) to demonstrate the retina layers. RPE, retinal pigmented epithelium; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar = 20 μm. C: Representative ultrastructure of connecting cilia emanating from heterozygous control (left panel) and ^retDync1h1^−/− basal bodies (right panel) at P6. Note the presence of the daughter centriole (DC), basal body (BB) docking to the membrane, and connecting cilium (CC) elaboration in the ^retDync1h1^−/− photoreceptor, scale bar = 0.3 μm. D: Axonemes and connecting cilia are absent at P8, scale bar = 0.6 μm. Left panel, heterozygous control; right panel, Dync1h1 knockout mouse. Modified from [72] with permission from PLOS One.