Figure 4. Protein conformational change study of monomers, oligomers, and fibrils. A: Tryptophan fluorescence assay. B: 8-anilino-1-naphthalene-sulfonic acid (ANS) binding assay of monomers, oligomers, and fibrils. The concentration of monomers, oligomers, and fibrils was 2.75 µM in both experiments. n = 3 (n represents number of times an experiment was repeated).