Figure 1. Aggregation kinetics of γD-crystallin under UV-C light. Reversed-phase high-performance liquid chromatography (RP-HPLC) sedimentation assay (blue), light scattering (red), and ThT binding (black) of γD-crystallin protein (3 µM) under ultraviolet (UV)-C light. Inset shows the aggregation reaction up to 2 h. In case of the RP-HPLC sedimentation assay, % monomers were converted to % aggregates, while the intensity values of light scattering and ThT binding assay are normalized considering intensity value at 18 h as 100%. n = 3 (n represents the number of times an experiment was repeated).