Figure 5. AMCM digested with proteinase K reverted inhibition of synthesis of chemokines in poly I:C–stimulated HLMs. Human limbal myofibroblasts
(HLMs) stimulated with polyinosinic-polycytidylic acid sodium salt (poly I:C) and incubated with amniotic membrane conditioned
medium (AMCM) statistically significantly decreased expression of CCL2, CCL5, and CXCL10; in contrast, proteinase K-treated
AMCM (AMCM+PK) statistically significantly reverted inhibition of synthesis of CCL2, CCL5, and CXCL10. Bars represent the
mean ± standard error (SE); ***p<0.001. All assays were performed three times in triplicate.