Figure 3. AMCM increased translocation of nuclear IRF3 and p-IRF3 and synthesis and secretion of IFN-β in poly I:C–stimulated HLMs.
A: Amniotic membrane conditioned medium (AMCM) statistically significantly increased the presence of nuclear and cytoplasmic
IRF3 and p-IRF3 in polyinosinic-polycytidylic acid sodium salt (poly I:C)–stimulated human limbal myofibroblasts (HLMs). The
medium fluorescence intensity (MFI) in each condition was achieved as described in the Materials and Methods section. B: AMCM statistically significantly increased the presence of IRF3 in the nucleus and cytoplasm in poly I:C–stimulated HLMs.
C: AMCM statistically significantly increased p-IRF3 in the nucleus and the cytoplasm in poly I:C–stimulated HLMs. Moreover,
AMCM increased IFN-β synthesis (D) and secretion (E) in poly I:C–stimulated HLMs. Bars represent the mean ± standard error (SE); ***p<0.001, **p<0.01; *p<0.05. All assays were
performed three times in triplicate. Scale bars = 50 μm.