Figure 2. AMCM inhibited translocation of nuclear NF-κB and p-NF-κB in poly I:C–stimulated HLMs. A: Polyinosinic-polycytidylic acid sodium salt (poly I:C) induced translocation of nuclear NF-κB and p-NF-κB in human limbal myofibroblasts (HLMs), which was inhibited in the presence of amniotic membrane conditioned medium (AMCM). The medium fluorescence intensity (MFI) in each condition was achieved as described in the Materials and Methods section. Poly I:C statistically significantly increased the presence of nuclear and cytoplasmic NF-κB and p-NF-κB; these protein increases were statistically significantly abolished by AMCM in the cytoplasm and nucleus subcellular compartments in the poly I:C–stimulated HLMs (B and C). Bars represent the mean ± standard error (SE); ***p<0.001; **p<0.01. All assays were performed three times in triplicate. Scale bars = 50 μm.