Figure 5. Piezo2 function in regulating aqueous humor dynamics. A: No statistically significant change in the intraocular pressure (IOP) was detected in the Piezo2^fl/fl mice receiving adenovirus 5 (Ad5)-Cre (13.4±2.90 mmHg, n = 45) compared to mice receiving Ad5-Con (13.9±2.70 mmHg, n = 34) during the first 2 weeks of injection. B: At the second week of injection, the outflow facility in mice receiving Ad5-Cre (0.0176±0.0100 μl/min/mmHg, n = 18) showed no statistically significant difference compared to the facility in mice receiving Ad5-Con (0.0139±0.0080 μl/min/mmHg, n = 16, p = 0.22). C: Two weeks after injection, the intercept of the linear regression line in the flow rate-pressure plot was decreased dramatically in the Piezo2^fl/fl mice receiving Ad5-Cre (0.09±0.17 µl/min, n = 18) compared to the Ad5-Con injected mice (0.20±0.11 µl/min, n = 14, p = 0.04). *p<0.05, p values were obtained from a two-tailed t test.