Table 2 of
Benitez-del-Castillo, Mol Vis 2021; 27:243-261. Table 2 of
Benitez-del-Castillo, Mol Vis 2021; 27:243-261.
Table 2. Tear sample volume required (per patient) for quantification with standard ELISA and multiplex antibody microarrays.
| Biomarker | ELISA | Microarrays | ||||
|---|---|---|---|---|---|---|
| Dilution | Final volume per well (µl) | Vol. sample needed* (µl) | Dilution | Final volume per well (µl) | Vol. sample needed** (µl) | |
| CST4 | 1:100 | 100 | 2 | 1:70 | 70 | 1 |
| MMP9 | 3:100 | 100 | 6 | |||
| S100A6 | 2:100 | 100 | 4 | |||
| S100A8 | 2:100 | 100 | 4 | N/A | N/A | N/A |
| Total volume (µl) | 16 | Total volume (µl) | 1 | |||