Figure 4. The optimised Kozak consensus achieved after refining the retina gene panel. Nucleotide frequencies using WebLogo 3.7.4 were achieved for positions -9 to -1 relative to the start codon. A standard GFP transcript sequence was used to compare the influence of the -9 to -1 nucleotides of 38 genes with known retina-specific roles. The identified preferential -9 to -1 nucleotide consensus of ACCGAGACC is in contrast to the classic consensus of GCCGCCACC (as in Figure 1).