Figure 2. Depiction of the reference GFP transcript. A standard GFP transcript sequence was used to compare the influence of the -9 to -1 nucleotides of 75 genes with known retina-specific functions on mRNA secondary structure minimum free energy (MFE) predictions using RNAfold (listed in Appendix 2). UTR = untranslated region; GFP = green fluorescent protein; WPRE = woodchuck hepatitis virus post-transcriptional regulatory element.