Figure 5. Cycloheximide chase of WT and P388S TULP1. A, B: Western blots of wild-type (WT) and P388S TULP1 enhanced green fluorescent protein (eGFP) stability in human embryonic kidney (HEK)-293A cells treated with 25 μM cycloheximide (CHX) and harvested at the indicated time points. C: Quantification of western blot from (A) and (B) showing percentage of TULP1 remaining over time when treated with CHX. (●) indicates WT TULP1 eGFP, and (■) indicates P388S TULP1 eGFP. n = 3 independent experiments, mean ± standard deviation (SD); *p<0.05, **p<0.01, two-tailed t test compared to each WT value, n.s., not significant.