**Figure 4. **Characterization of the P388S TULP1 variant. **A**: Western blot of wild-type (WT) and P388S TULP1 enhanced green fluorescent protein (eGFP) levels in soluble and insoluble
fractions. **B**: Quantification of WT and P388S TULP1 eGFP expression in soluble and insoluble fractions of western blot in (**A**), n≥5, mean ± standard deviation (SD; **p<0.01, one-sample *t* test versus hypothetical value of 1 [i.e., unchanged]). **C**: Quantitative PCR (qPCR) of TULP1 mRNA expression from WT TULP1 eGFP- and P388S TULP1 eGFP-transfected HEK-293A cells. Representative
data of n≥3 independent experiments, mean ± SD; n.s., not significant.