Table 3 of
Bouzidi, Mol Vis 2021; 27:17-25. Table 3 of
Bouzidi, Mol Vis 2021; 27:17-25.
Table 3. Primer sequences and annealing temperature for Sanger sequencing.
| Targeted gene (Family) | Exon | Base sequence | Annealing temperature °C |
|---|---|---|---|
| CRB1 | 6a | F: 5’ CAGGTCAATAATCAGTCAAAGG 3’ | 58 |
| (RP1) | R: 5’ CAAACGAAGGTGTGGATGGC 3’ | ||
| 6b | F : 5’ ACCAGTGGGAATGACCAGC 3’ | 60 | |
| R: 5’ CTGTGGCAGTCACACTGG 3’ | |||
| PDE6B | 15 | F: 5’ AGAGGCAGACCCTGTCTCAA 3’ | 58 |
| (RP2) | R: 5’ CAGTGCTCACACACCCAGTG 3’ | ||
| ABCA4 | 43 | F: 5’ GCTCACACACACACTTACCC 3’ | 58 |
| (RP3) | R: 5’GACCTGTGAGAGACTCCCTG 3’ | ||
| 45 | F: 5’ TTCTGGAGGCGTGAGATCTG 3’ | 62 | |
| R: 5’ TTGGTTTAAGCCCTTGGTGC 3’ |