Figure 3. Gene expression of NADPH oxidase enzyme. Relative mRNA expression of (A) NOX1, (B) NOX2, (C) NOX4, (D) p22phox, and (E) p47phox, in the eyes of normotensive (CONTROL) and hypertensive (Nω-nitro-L-arginine methyl ester, L-NAME) rats. The quantitative fold changes in gene expression were determined as relative to GAPDH in each corresponding group and calculated using the 2^-ΔΔCt formula. Values are expressed as mean ± standard error of the mean (SEM) of seven animals per group. **p<0.01, ***p<0.001 versus the CONTROL group.