Figure 1. Cloning of VMA and iHA-VMA. A: Schematic representation of the constructs, Vibrio mimicus collagenase that remains active (VMA) and inhibitor of hyaluronic acid-VMA (iHA-VMA). B: Agarose gel showing the PCR amplicons (about 1.5 kb) corresponding to iHA-VMA and VMA, respectively, and 100 bp ladder.
C: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) profile of the purified proteins, VMA and iHA-VMA.