Appendix 3 of Santra, Mol Vis 2021; 27:125-141.

Appendix 3. Assessment of binding of iHA-VMA with HA.

To access the data, click or select the words “Appendix 3.” A: Diagrammatic representation of binding of HA-FITC to CD44 cell surface receptor on ARPE-19 cells, in the presence or absence of iHA-VMA; FITC labeled HA is expected to bind to CD44, resulting in strong signal of the dye, while incubation of HA-FITC with iHA-VMA, is expected to inhibit the binding of HA to CD44 (with a reduction in signal intensity of the dye), due to strong binding of the 12-mer iHA to HA. B: Raw data of the flow cytometry experiment, wherein ARPE-19 cells were gated in forward/side scatter; histograms showing the peaks corresponding to mean fluorescence intensity (MFI) of cells alone or binding of indicated proteins to ARPE-19 cell surface CD44 receptor.