Table 2 of Nuzzi, Mol Vis 2020; 26:818-829.

Table 2. Details of the primers used for qRT-PCR technique.

Gene Forward primer (5’-3’) Reverese primer (5’-3’) References
hCD45 CTGACATCATCACCTAGCAG TGCTGTAGTCAATCCAGTGG [37]
hCD11a TGAGAGCAGGCTATTTGGGTTAC CGGCCCATGTGCTGGTAT [38]
hCD64 GCCACAGAGGATGGAAATGT CATGAAACCAGACAGGAGTGG [39]
hCD163 TCAGAGTTTCAACCAGAAGGAG TGTCATCTGAGGTTCTTGTCC [40]
hCD68 TGGATTCATGCAGGACCTCC CGCCATGTAGCTCAGGTAGACA [41]
hCD11b GGCCATGCACAGATACCAGGT CTGGGGGTGCGATTTTCT [41]
hCD16 TCGAGCTACTTCATTGACGC GATATGGACTTCTAGCTGCACC [42]
H vimentin AGGAAATGGCTCGTCACCTTCGTGAATA GGAGTGTCGGTTGTTAAGAACTAGAGCT [43]
hGFAP ACATCGAGATCGCCACCTAC ACATCACATCCTTGTGCTCC [44]
hRlbp GCTGCTGGAGAATGAGGAAACTC GGCTGGTGGATGAAGTGGAT [45]
B2M AGCAAGGACTGGTCTTTCTATCTC ATGTCTCGATCCCACTTAACTA [46]

Abbreviations: hGFAP, human glial fibrillary acidic protein; hRlbp, human retinaldehyde binding protein 1; B2M, beta-2-Microglobulin used as reference gene to normalize the cDNA in different samples; qRT-PCR, quantitative real-time polymerase chain reaction.

Nuzzi, Mol Vis 2020; 26:818-829. http://www.molvis.org/molvis/v26/818

©2020 Molecular Vision http://www.molvis.org/molvis/

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