Figure 3. Hyaluronic acid assay. Hyaluronic acid was quantified in supernatants from primary culture hyalocytes after 6 days of treatment with 10 ng/ml basic fibroblast growth factor (bFGF), 5 ng/ml transforming growth factor beta 1 (TGF-β), 10 ng/ml platelet-derived growth factor subunit-BB (PDGF-BB), 200 μg/ml ascorbic acid (AsA), 1 mg/ml dexamethasone (DEX) and 5 μM H2O2., and in media from A549 cells and human fibroblasts. Measurements were performed in triplicate; data were represented as means ± standard deviation (SD); The analysis of variance (ANOVA) with Tukey’s post-hoc correction was performed. *p<0.001 versus untreated (NT) primary cultured hyalocytes.