Table 2 of
Schnabel, Mol Vis 2020; 26:797-817. Table 2 of
Schnabel, Mol Vis 2020; 26:797-817.
Table 2. Antibodies used in immunocytochemistry (ICC), immunohistochemistry (IHC), and western blot (WB) analysis.
| Method | Antibody | Source | Catalog # | Dilution Concentration |
|---|---|---|---|---|
| ICC | rabbit anti-human AQP8 | antibodies-online, Aachen, Germany | ABIN571717 | 1:500 2 µg/ml |
| rabbit anti-human Kir6.1 | Invitrogen, Paisley, UK | ThermoFisher Scientific, Waltham, MA ‒ PA5–56628 | 1:200 0.5 µg/ml | |
| monoclonal mouse anti-human cytochrome c | Santa Cruz Biotechnology, Heidelberg, Germany | ThermoFisher Scientific ‒ sc13156 | 1:50 4 µg/ml | |
| monoclonal mouse anti-human ZO-1 | Invitrogen | 33–9100 | 1:100 5 µg/ml | |
| Cy3-coupled goat anti-rabbit IgG | Jackson Immuno Research, West Grove, PA | 111–165–003 | 1:200 10 µg/ml | |
| Alexa Fluor 488-coupled goat anti-mouse IgG | Invitrogen | ThermoFisher Scientific ‒ A-11001 | 1:200 10 µg/ml | |
| IHC | rabbit anti-human AQP8 | antibodies-online | ABIN571717 | 1:500 2 µg/ml |
| monoclonal mouse anti-human ZO-1 | Invitrogen | 33–9100 | 1:50 10 µg/ml | |
| monoclonal mouse anti-human cytochrome c | Santa Cruz Biotechnology | sc13156 | 1:50 4 µg/ml | |
| Alexa 568-coupled goat anti-rabbit IgG | Invitrogen | A11036 | 1:400 5 µg/ml | |
| Alexa 647-coupled goat anti-mouse IgG | Invitrogen | A21236 | 1:200 10 µg/ml | |
| WB | rabbit anti-human AQP8 | antibodies-online | ABIN571717 | 1:1000 1 µg/ml |
| rabbit anti-β-actin | Cell Signaling Technology, Frankfurt/M., Germany | 4970 | 1:1000 37 ng/ml | |
| alkaline phosphatase-linked anti-rabbit IgG | Cell Signaling Technology | 7054S | 1:2000 35.5 ng/ml |