Figure 3 of Schnabel, Mol Vis 2020; 26:797-817.

Figure 3. Intracellular signaling involved in mediating NaCl-induced expression of the AQP8 gene in RPE cells. The level of AQP8 mRNA was determined with real-time RT–PCR analysis in cells cultured for 6 h in iso- (control) and hyperosmotic (+ 100 mM NaCl) media (as indicated by the panels of the bars), and is expressed as folds of unstimulated control. The following agents were tested: the inhibitor of ERK1/2 activation, PD98059 (20 µM), the inhibitor of p38 MAPK activation, SB203580 (10 µM), the JNK inhibitor SP600125 (10 µM), the inhibitor of PI3K-related kinases, LY294002 (5 µM), the JAK2 inhibitor AG490 (10 µM), the PLCγ inhibitor U73122 (4 µM), the inhibitor of PKCα/β, Gö6976 (1 µM), the SGK inhibitor GSK650394 (GSK; 1 µM), and the PLA2 inhibitor 4-bromophenacyl bromide (Bromo; 300 µM). Each bar represents mean ± standard error of the mean (SEM) obtained in three to 16 independent experiments using cell lines from different donors. Significant difference vs. unstimulated control: *p<0.05. Significant difference vs. NaCl control: p<0.05.