To access the data, click or select the words “Appendix 3.” (A) Cultured Primary Bovine Retinal Endothelial cells (RMECs) were dissociated. Cells were captured on the Fluidigm C1 system and lysed, mRNA reverse transcribed to cDNA then PCR amplified using the Clontech SmartSeq-V4 protocol. (B) cDNA libraries were prepared using a miniaturised Nextera XT library prep protocol and sequenced on an Illumina NextSeq 500, using a 150 cycle (2×75 bp) NextSeq kit. (C) Bioinformatic analysis was performed using Python and R programs and the web-based automated single cell analysis pipeline ‘ASAP’.