Table 1 of Gupta, Mol Vis 2020; 26:742-756.

Table 1. Sequence of primers used in the study.

Gene Name Forward Primer (5′ − 3′) Reverse Primer (5′ − 3′) Accesion No. Species Amplicon size (bp)
For Real Time PCR
β-actin CGGCTACAGCTTCACCACCA CAGGCAGCTCGTAGCTCTTC X_00351 Human 143
α-Smooth muscle actin TGGGTGACGAAGCACAGAGC CTTCAGGGGCAACACGAAGC NM_001613 Human 138
Fibronectin CGCAGCTTCGAGATCAGTGC TCGACGGGATCACACTTCCA NM_002026 Human 142
Collagen I TGTGGCCCAGAAGAACTGGTACAT ACTGGAATCCATCGGTCATGCTCT NM_001845 Human 88
Collagen IV AGGTGTTGACGGCTTACCTG TTGAGTCCCGGTAGACCAAC NM_000088.3 Human 132
For Regular PCR
β-actin AGGCCAACCGCGAGAAGATGACC GAAGTCCAGGGCGACGTAGCAC X_00351 Human 350
Id3-mCherry CGCGTCATCGACTACATTCTC CCCATGGTCTTCTTCTGCATT NA NA 617
Id3 ATTAAGCTTGCCACCATGAA
GGCGCTGAGCCCGGTGCGCG TCGGGATCCTGGCAAAAGCTCCT
TTTGTCGTTGGAGATGAC NM_002167 Human 390

Note: Sequence of the forward and reverse primers were used in the study to confirm the expression of different proteins on mRNA level using RT–PCR or to confirm the expression of protein through regular PCR amplification. bp=basepairs; NA=not applicable.

Gupta, Mol Vis 2020; 26:742-756. http://www.molvis.org/molvis/v26/742

©2020 Molecular Vision http://www.molvis.org/molvis/

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