Figure 1. Agarose gel electrophoresis showing successful delivery of the Id3-mCherry gene into h-CSFs. An amplified Id3-mCherry gene product at 617 bp in Id3-mCherry human stromal corneal fibroblasts (h-CSFs; lane 3) was noted but not in the normal (lane 3) or naked-vector transfected (lane 3) h-CSFs. Negative controls containing Id3-mCherry forward and reverse primers without cDNA showed no amplification product (lane 1). The quality of the cDNA and PCR reagents was confirmed using β-actin forward and reverse primers (lane 2). The DNA ladder was 100 bp.