Figure 1. Dissociation of the retina for scRNA-seq. A: Flowchart showing different steps of the two protocols for preparing retina cell suspensions. B: Vertical section showing EGFP (green) and tdTomato (pseudo-colored in magenta) expression in rods and ON bipolar cells,
respectively, in the Nrl-L-EGFP/Grm6-tdTomato transgenic mouse retina. OS: outer segment; IS: inner segment; ONL: outer nuclear layer; OPL: outer plexiform layer; INL:
inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. C: Representative images of cell suspensions derived from the Nrl-L-EGFP/Grm6-tdTomato transgenic mouse retina following a frequently used protocol and the optimized protocol for single-cell RNA sequencing (scRNA-seq)
sample preparation. D: Higher magnification images of rods, cones, and bipolar and Müller cells obtained with the optimized protocol.