Figure 2. Treatment with tofacitinib selectively inhibits production of IFN-γ, but not of IL-17. A: Cytokine levels in culture supernatants. Spleen cells from B10.A mice immunized with interphotoreceptor retinoid-binding protein (IRBP) and treated with tofacitinib, or vehicle, were collected on day 14 post-immunization and cultured with IRBP or purified protein derivative (PPD) at the indicated concentrations. Supernatants collected after 48 h incubation were analyzed for the levels of interleukin (IL)-17 and interferon (IFN)-γ using enzyme-linked immunosorbent assay (ELISA) kits. The data combine actual values of three separate experiments, using the formula shown in reference 14. *p<0.05; **p<0.01. B and C: Intracellular cytokine staining for IFN-γ and IL-17, or their transcriptional factors, T-bet and RORγt, in mouse eye-infiltrating (B) and spleen (C) cells, collected on day 14 post-immunization. The methods are detailed in the Materials and Methods section. On the left are shown actual flow cytometric results of representative experiments and on the right are summaries of four independent experiments ± standard error of the mean (SEM), with five mice per group. Statistical significance was determined using an unpaired t test. *p<0.05, ** p<0.001, versus vehicle group.