Figure 3. Verification of POAG-associated alterations in the content of the major proteins of the human sclera. A: Representative western blotting images of thrombospondin-4 (THBS4), vimentin (VIM), angiopoietin-related protein 7 (ANGPTL7), annexin A2 (ANXA2), and serum amyloid P component (APCS) in protein extracts obtained from non-glaucomatous sclera (control) and sclera of patients with different stages of primary open-angle glaucoma (POAG; glaucoma). The amount of the total protein in each track is adjusted using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. Arrows indicate apparent molecular weights (in kDa) of the proteins. B: The weight fractions of the scleral proteins estimated from the western blotting data obtained for control individuals (100%) and patients with different stages of POAG. *p<0.05 compared to the values obtained for the control group. The actual p values for all pairwise comparisons are given in Appendix 7.