Appendix 3 of Li, Mol Vis 2020; 26:603-612.
To access the data, click or select the words “Appendix 3.” Confocal fluorescent images show no CP49 proteins in lens fibers at zones I (A), II (B) and III (C) from 37 °C, 25 °C and 4 °C treated 129WT lenses. The lens cross-sectional samples were immunolabeled with anti-CP49 antibody (red), FITC-phalloidin (green) and DAPI (blue); the left panels are CP49 alone and the right panels are merged with F-actin and nuclei. (D) Enlarged images of the white-boxed regions in zone III (C). Scale bar: A-C, 10 μm; D, 4 μm.