To access the data, click or select the words “Appendix 2.” Confocal fluorescent images show extremely low levels of filensin proteins in lens fibers at zones I (A), II (B) and III (C) of 37 °C, 25 °C and 4 °C treated 129WT lenses. The lens cross-sectional samples were immunolabeled with anti-filensin antibody (red), FITC-phalloidin (green) and DAPI (blue); the left panels are filensin alone and the right panels are merged with F-actin and nuclei. (D) Enlarged images of the white-boxed areas in zone III in (C). Scale bar: A-C, 10 μm; D, 4 μm.