Figure 3. Myoc-N450Y regulates cell viability and apoptosis in primary HTM cells. A: Cell viability was detected with Cell Counting Kit-8 (CCK-8). **p<0.05. B: The protein level of P21 and P27 in primary human trabecular meshwork (HTM) cells with Myoc-WT or Myoc-N450Y overexpression.
C: Myoc-WT or Myoc-N450Y overexpression was subjected to flow cytometry analysis of apoptosis. **p<0.05. D: Western blotting was used to detect the expression of apoptosis-related proteins. Data were quantified from three independent
experiments (n = 3).