Figure 2. Aggregation of Myoc-N450Y induces ER stress and activates UPR. A: No myocilin secretion in the Myoc-N450Y group. B: Myoc-N450Y accumulates in the endoplasmic reticulum (ER) of primary human trabecular meshwork (HTM) cells. Colocalization of Myoc (Red) with CALR (green) was examined with immunofluorescence. C: Increased mRNA levels of ER stress markers in the Myoc-N450Y group. *p<0.01, **p<0.05, ***p<0.001. D: Increased protein level of ER stress markers in the Myoc-N450Y group. Data were quantified from three independent experiments (n = 3).