Figure 1. Characterization and overexpression of Myoc-WT and Myoc-N450Y in primary HTM cells. A: Phase contrast microscopic images of human trabecular meshwork (HTM) cells. B: Expression of TIMP3 and LAMA4 in HTM cells at passage 2 (P2), passage 4 (P4), and passage 6 (P6). C: Myocilin expression in HTM cells with or without dexamethasone treatment. D: mRNA levels of Myoc-WT and Myoc-N450Y were elevated in cultured primary HTM cells. ***p<0.001. E: Protein levels of Myoc-WT and Myoc-N450Y were elevated in cultured primary HTM cells. Data were quantified from three independent
experiments (n = 3).