Figure 6. IL-6 is dramatically upregulated and JAK1/STAT3 signaling is activated in the mouse PVR model in vivo. The mouse model of proliferative vitreoretinopathy (PVR) was induced with intravitreal injection of 1 μl of dispase/collagenase at the concentration of 0.02 U/μl in the right eye with a 30 G Hamilton syringe. A: Retinas were harvested for quantitative real-time PCR analysis of interleukin-6 (IL-6) and gp130 mRNA levels at day 1, 3, 7, and 14 after injection. B: Western blotting of the expression of IL-6 and gp130 protein levels at day 1 after injection. C: Quantification of IL-6 and gp130 protein levels from three independent experiments. P values versus the control group are shown (Student t test). D: Western blotting of the expression of p-JAK1, JAK1, p-STAT3, and STAT3 levels at day 1 after injection. Quantification of the p-JAK1, JAK1, p-STAT3, and STAT3 protein levels from three independent experiments. E: P values versus the control group are shown (Student t test).