Figure 3 of Chen, Mol Vis 2020; 26:517-529.


Figure 3. JAK1/STAT3 signaling is activated by IL-6 in RPE cells. RPE cells were cultured with various concentrations of interleukin-6 (IL-6; 5, 10, 25, and 50 ng/ml) for 60 min. A: The protein expression of p-JAK1, JAK1, p-STAT3, and STAT3 was determined with western blotting. B: Quantification of p-JAK1, JAK1, p-STAT3, and STAT3 protein levels from three independent experiments. RPE cells were cultured with 25 ng/ml of IL-6 for different times, and p-JAK1, JAK1, p-STAT3, and STAT3 expression was determined with western blotting. C: Representative results from one of the three independent experiments are presented. D: Quantification of p-JAK1, JAK1, p-STAT3, and STAT3 protein levels from three independent experiments.