Figure 7. Western blotting analyses of expression of Nrf2, HO-1 and Bax in retinas of mice treated with NaIO3 or NaIO3 + TMP at three time points. A: Western blot detection of Nrf2, HO-1, and BAX protein expression levels of the three groups. B: Nrf2 expression levels increased by approximately twofold and threefold in the tetramethylpyrazine (TMP) group at day 3
and 7 post-NaIO3 injection, respectively, when compared with the control group. The increase in the TMP group was more obvious than that in
the NaIO3-degenerated group (Group I) at day 3 and 7 post-NaIO3 injection. C: HO-1 expression levels were approximately twofold in the TMP group at day 3 and 7 post-NaIO3 injection when compared with those of the control group. There were statistically significant differences among the three
groups at the three time points, and the expression levels in the TMP group were higher than those of Group I at day 3 and
7 post-NaIO3 injection. D: BAX expression levels increased by approximately sixfold in the TMP group and Group I at day 3 day post-NaIO3 injection when compared with the control group. It decreased gradually in the TMP group in the following days but remained
high in Group I for 14 days. The Bax expression levels in Group I were statistically significantly higher than those of the
TMP group. (n = 3,*p<0.05, **p<0.01).