Figure 5. TUNEL assays photographs of mice treated with NaIO3 or NaIO3 + TMP at three points. Top: Group C. The second to fourth arrays are the NaIO3-degenerated group (Group I) at day 3, 7, and 14 after NaIO3 administration. The fifth to seventh arrays are the tetramethylpyrazine (TMP) group at day 3, 7, and 14 after NaIO3 administration. Left to right: Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), 4′,6-diamidino-2-phenylindole
(DAPI), and Merged. Group C: TUNEL-positive cells were not found in Group C. I3d: Multiple of TUNEL-positive cells (green)
were found in the ONL. I7d: TUNEL-positive cells were decreased in the ONL. I14d: Few TUNEL-positive cells were found in the
ONL. TMP3d: Many TUNEL-positive cells were observed in the ONL. TMP7d: TUNEL-positive cells were decreased in the ONL. TMP14d:
Only a few TUNEL-positive cells were found in the ONL. White arrows mark TUNEL-positive cells. Scale bar: 50 μm.