Figure 5. TUNEL assays photographs of mice treated with NaIO₃ or NaIO₃ + TMP at three points. Top: Group C. The second to fourth arrays are the NaIO₃-degenerated group (Group I) at day 3, 7, and 14 after NaIO₃ administration. The fifth to seventh arrays are the tetramethylpyrazine (TMP) group at day 3, 7, and 14 after NaIO₃ administration. Left to right: Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), 4′,6-diamidino-2-phenylindole (DAPI), and Merged. Group C: TUNEL-positive cells were not found in Group C. I3d: Multiple of TUNEL-positive cells (green) were found in the ONL. I7d: TUNEL-positive cells were decreased in the ONL. I14d: Few TUNEL-positive cells were found in the ONL. TMP3d: Many TUNEL-positive cells were observed in the ONL. TMP7d: TUNEL-positive cells were decreased in the ONL. TMP14d: Only a few TUNEL-positive cells were found in the ONL. White arrows mark TUNEL-positive cells. Scale bar: 50 μm.