Figure 5. Efficiency of gene knockdown with Invivofectamine 3.0. A: In vivo-jetPEI^®:Gapdh siRNA produced no statistically significant change in gene Gapdh expression 1 day post-injection when compared to controls whereas Invivofectamine 3.0 showed a statistically significant knockdown. B: Invivofectamine 3.0:Gapdh siRNA produced statistically significant targeted gene knockdown of Gapdh at 1 and 3 days post-injection. Five and 7 days showed no statistically significant difference compared to negative siRNA controls. C: Invivofectamine 3.0 complexed with siRNA against the inflammatory chemokine Il-1β also produced statistically significant knockdown. D: Invivofectamine 3.0:C3 siRNA allowed for a statistically significant gene knockdown as well (*p<0.05 using one-way ANOVA with Tukey’s post hoc test (A) and unpaired Student t test (C, D), n=4–11).