Figure 5. Dose-dependent increase in NO levels in human corneoscleral segments after treatment with latanoprostene bunod compared with latanoprost vehicle control. Cultured human donor corneoscleral segments were treated with increasing doses of latanoprostene bunod (5–20 μM) or equivalent volume of latanoprost (vehicle), and changes in the levels of NO from baseline were recorded. Each subsequent bolus dose was given after the signal plateaued (~10 min). Washout readings were recorded after a 30-min period. Data are expressed as means ± standard error of the mean (SEM); n = 4 for each group; ** p<0.01, *** p<0.001; two-way analysis of variance (ANOVA).