Figure 3. Immunofluorescence analysis on cross sections. A: Exemplary images of cross sections obtained from the buffer-treated (left), control vector-NMDA-treated (middle), and therapeutic vector-NMDA-treated (right) groups. Red: Brn3a, Blue: DAPI. Images were captured at a distance of 1,000 µm from the ONH. Scale bar represents 100 μm. B: Total number of Brn3a-positive cells in GCL from one ora serrata to the other (n = 3 in each group, *p<0.05 in SNK test).