Figure 2. The PGE2 receptors are involved in the regulation of the EGF-dependent activation of astrocytes. A: The morphological changes in astrocytes in response to epidermal growth factor (EGF). Astrocytes were treated without EGF or with 40 ng/ml EGF for 24 h (10X). The scale bar represents 50 μm. B-D: EGFR and glial fibrillary acidic protein (GFAP) were increased after treatment with EGF. Cells were immunoblotted with the EGFR and GFAP antibodies for immunoblotting. E- I: The expression level of EP1–4 was evaluated with western blotting. β-tubulin was used as a loading control in all samples. Data were shown as mean ± standard error of the mean (SEM; *p<0.05, **p<0.01, n=6).