Figure 4. Immunofluorescence staining for GFAP in the rat retina. A: Representative images of immunofluorescence staining for GFAP (red), counterstained with 4′, 6-diamidino-2-phenylin-dole (DAPI; blue) for cellular nuclei. Magnification = 400X. Scale bar = 50 µm. B: Quantification of GFAP staining. Data are mean ± standard error of the mean (SEM, n=3). C, control; D, diabetes; D+B12, diabetic rats treated with vitamin B₁₂. **Significant difference from the control group; ^#significant difference from the diabetes group (p<0.05).