Table 1 of Shivashankar, Mol Vis 2020; 26:277-290.
Incubation condition | Incubation buffer | Supplement to incubation buffer | Gas mixture | Phenotype |
---|---|---|---|---|
Control | Normal physiological buffer | - | 95% O2 & 5% CO2 | Normal |
High glucose | Normal physiological buffer | 20 mM Glucose | 95% O2 & 5% CO2 | Hyperglycemia |
High glucose + proinflammatory cytokines | Normal physiological buffer | 20 mM Glucose, 10 ng/ml IL-1β, 10 ng/ml TNF- α | 95% O2 & 5% CO2 | Hyperglycemia with inflammation |
D- Mannitol | Normal physiological buffer | 20 mM D- Mannitol | 95% O2 & 5% CO2 | Osmotic control |
Proinflammatory cytokines | Normal physiological buffer | 10 ng/ml IL-1β, 10 ng/ml TNF- α | 95% O2 & 5% CO2 | Cytokines control |
Anoxia | Normal physiological buffer | - | 95% N2 & 5% CO2 | Cellular damage control |