Table 1 of Shivashankar, Mol Vis 2020; 26:277-290.


Table 1. In vitro incubation conditions used in this study.

Incubation condition Incubation buffer Supplement to incubation buffer Gas mixture Phenotype
Control Normal physiological buffer - 95% O2 & 5% CO2 Normal
High glucose Normal physiological buffer 20 mM Glucose 95% O2 & 5% CO2 Hyperglycemia
High glucose + proinflammatory cytokines Normal physiological buffer 20 mM Glucose,
10 ng/ml IL-1β,
10 ng/ml TNF- α 95% O2 & 5% CO2 Hyperglycemia with inflammation
D- Mannitol Normal physiological buffer 20 mM D- Mannitol 95% O2 & 5% CO2 Osmotic control
Proinflammatory cytokines Normal physiological buffer 10 ng/ml IL-1β,
10 ng/ml TNF- α 95% O2 & 5% CO2 Cytokines control
Anoxia Normal physiological buffer - 95% N2 & 5% CO2 Cellular damage control