Appendix 2 of Shivashankar, Mol Vis 2020; 26:277-290.

Appendix 2. The four immunogold labeled images used to quantify glutamate labeling in retinal explants.

To access the data, click or select the words “Appendix 2.” Glutamate labeling in control (A-D), high glucose alone (E-H) and high glucose in combination with proinflammatory cytokines (I-L) condition. The retinal layers of interest are indicated on the left-hand side of image A. The two horizontal lines on the left-hand side of each image identify the IPL. Cells of interest are indicated in image A with the bipolar cell, identified in the INL close to the OPL (BC-red arrow), the stellar-shape soma of Müller cells in the middle of the INL (MC-blue arrow), the amacrine cells in the lower row of the INL adjacent to the IPL (AC-yellow arrow), the cells in the ganglion cell layer (GC-orange arrow), and the intercellular space corresponding to Müller cell endfeet in the GCL (MCef- black arrowhead). Glutamate labeling in MCef (arrowhead and insert) displayed increased labeling in high glucose alone (E-H), and in combination with proinflammatory cytokines (I-L). Coapplication of high glucose and proinflammatory cytokines also displayed decreased glutamate labeling in bipolar cells (red arrows in I-H). Scale bar is equal to 25 µm. Abbreviations: OS-photoreceptor outer segment; IS-photoreceptor inner segment; ONL-outer nuclear layer; OPL-outer plexiform layer; INL- inner nuclear layer; IPL-inner plexiform layer; GCL-ganglion cell layer.