Appendix 2 of Shivashankar, Mol Vis 2020; 26:277-290.
To access the data, click or select the words “Appendix 2.” Glutamate labeling in control (A-D), high glucose alone (E-H) and high glucose in combination with proinflammatory cytokines (I-L) condition. The retinal layers of interest are indicated on the left-hand side of image A. The two horizontal lines on the left-hand side of each image identify the IPL. Cells of interest are indicated in image A with the bipolar cell, identified in the INL close to the OPL (BC-red arrow), the stellar-shape soma of Müller cells in the middle of the INL (MC-blue arrow), the amacrine cells in the lower row of the INL adjacent to the IPL (AC-yellow arrow), the cells in the ganglion cell layer (GC-orange arrow), and the intercellular space corresponding to Müller cell endfeet in the GCL (MCef- black arrowhead). Glutamate labeling in MCef (arrowhead and insert) displayed increased labeling in high glucose alone (E-H), and in combination with proinflammatory cytokines (I-L). Coapplication of high glucose and proinflammatory cytokines also displayed decreased glutamate labeling in bipolar cells (red arrows in I-H). Scale bar is equal to 25 µm. Abbreviations: OS-photoreceptor outer segment; IS-photoreceptor inner segment; ONL-outer nuclear layer; OPL-outer plexiform layer; INL- inner nuclear layer; IPL-inner plexiform layer; GCL-ganglion cell layer.